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Targeting Snail1 by CRISPR/Cas9 System Inhibits the Proliferation and Migration of Human Gastric Cancer Cells

Authors

  • Peng-Wei Zhang

    Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, MOE Key Laboratory of Tumor Molecular Biology, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China

  • Zhe-Sheng Chen

    Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, St. John’s University, Queens, New York 11432, USA

  • Zhi Shi

    Department of Cell Biology & Institute of Biomedicine, National Engineering Research Center of Genetic Medicine, MOE Key Laboratory of Tumor Molecular Biology, Guangdong Provincial Key Laboratory of Bioengineering Medicine, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China

DOI:

https://doi.org/10.30683/1929-2279.2023.12.5

Keywords:

Gastric cancer, Snail1, CRISPR/Cas9, gene editing, cancer proliferation, cancer migration

Abstract

The zinc-finger transcriptional repressor Snail1 affects cancer progression by controlling the epithelial cell-mesenchymal transition. The RNA-guided clustered regularly interspaced short palindromic (CRISPR) with a CRISPR-associated nuclease 9 (Cas9) nuclease system has been extensively used for gene editing. Here, we used two distinct sgRNAs to successfully target Snail1 in the gastric cancer cell line MGC803 with the CRISPR/Cas9 system. Furthermore, we discovered that Snail1 knockout reduced the proliferation and migration of MGC803 cells.

References

Stemmler MP, Eccles RL, Brabletz S, Brabletz T. Non-redundant functions of EMT transcription factors. Nat Cell Biol 2019; 21(1): 102-112. https://doi.org/10.1038/s41556-018-0196-y

Goossens S, Vandamme N, Van Vlierberghe P, Berx G. EMT transcription factors in cancer development re-evaluated: Beyond EMT and MET. Biochim Biophys Acta Rev Cancer 2017; 1868(2): 584-591. https://doi.org/10.1016/j.bbcan.2017.06.006

Hwang WL, Yang MH, Tsai ML, Lan HY, Su SH, Chang SC, Teng HW, Yang SH, Lan YT, Chiou SH, Wang HW. SNAIL regulates interleukin-8 expression, stem cell-like activity, and tumorigenicity of human colorectal carcinoma cells. Gastroenterology 2011; 141(1): 279-91, 291.e1-5. https://doi.org/10.1053/j.gastro.2011.04.008

Batlle E, Sancho E, Francí C, Domínguez D, Monfar M, Baulida J, García De Herreros A. The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat Cell Biol 2000; 2(2): 84-9. https://doi.org/10.1038/35000034

Qiao L, Gao H, Zhang T, Jing L, Xiao C, Xiao Y, Luo N, Zhu H, Meng W, Xu H, Mo X. Snail modulates the assembly of fibronectin via α5 integrin for myocardial migration in zebrafish embryos. Sci Rep 2014; 4: 4470 https://doi.org/10.1038/srep04470

Kajita M, McClinic KN, Wade PA. Aberrant expression of the transcription factors snail and slug alters the response to genotoxic stress. Mol Cell Biol 2004; 24(17): 7559-66 https://doi.org/10.1128/mcb.24.17.7559-7566.2004

Ikenouchi J, Matsuda M, Furuse M, Tsukita S. Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J Cell Sci 2003; 116(Pt 10): 1959-67. https://doi.org/10.1242/jcs.00389

Nieto MA. The snail superfamily of zinc-finger transcription factors. Nat Rev Mol Cell Biol 2002; 3(3): 155-66, https://doi.org/10.1038/nrm757

Lin Y, Wu Y, Li J, Dong C, Ye X, Chi YI, Evers BM, Zhou BP. The SNAG domain of Snail1 functions as a molecular hook for recruiting lysine-specific demethylase 1. EMBO J 2010; 29(11): 1803-16. https://doi.org/10.1038/emboj.2010.63

Peinado H, Olmeda D, Cano A. Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype? Nat Rev Cancer 2007; 7(6): 415-28. https://doi.org/10.1038/nrc2131

Wang H, La Russa M, Qi LS. CRISPR/Cas9 in Genome Editing and Beyond. Annu Rev Biochem 2016; 85: 227-64. https://doi.org/10.1146/annurev-biochem-060815-014607

Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Genome-scale CRISPR-Cas9 knockout screening in human cells. Science 2014; 343(6166): 84-87. https://doi.org/10.1126/science.1247005

Yuasa Y. Control of gut differentiation and intestinal-type gastric carcinogenesis. Nat Rev Cancer 2003; 3(8): 592-600. https://doi.org/10.1038/nrc1141

He H, Chen W, Wang X, Wang C, Liu F, Shen Z, Xu J, Gu J, Sun Y. Snail is an independent prognostic predictor for progression and patient survival of gastric cancer. Cancer Sci 2012; 103(7): 1296-303. https://doi.org/10.1111/j.1349-7006.2012.02295.x

Kato Y, Yashiro M, Noda S, Tendo M, Kashiwagi S, Doi Y, Nishii T, Matsuoka J, Fuyuhiro Y, Shinto O, Sawada T, Ohira M, Hirakawa K. Establishment and characterization of a new hypoxia-resistant cancer cell line, OCUM-12/Hypo, derived from a scirrhous gastric carcinoma. Br J Cancer 2010; 102(5): 898-907. https://doi.org/10.1038/sj.bjc.6605543

Ryu HS, Park DJ, Kim HH, Kim WH, Lee HS. Combination of epithelial-mesenchymal transition and cancer stem cell-like phenotypes has independent prognostic value in gastric cancer. Hum Pathol 2012; 43(4): 520-8. https://doi.org/10.1016/j.humpath.2011.07.003

Koh JS, Joo MK, Park JJ, Yoo HS, Choi BI, Lee BJ, Chun HJ, Lee SW. Inhibition of STAT3 in gastric cancer: role of pantoprazole as SHP-1 inducer. Cell Biosci 2018; 8: 50. https://doi.org/10.1186/s13578-018-0248-9

Shin NR, Jeong EH, Choi CI, Moon HJ, Kwon CH, Chu IS, Kim GH, Jeon TY, Kim DH, Lee JH, Park DY. Overexpression of Snail is associated with lymph node metastasis and poor prognosis in patients with gastric cancer. BMC Cancer 2012; 12: 521. https://doi.org/10.1186/1471-2407-12-521

Mirzaei S, Gholami MH, Aghdaei HA, Hashemi M, Parivar K, Karamian A, Zarrabi A, Ashrafizadeh M, Lu J. Exosome-mediated miR-200a delivery into TGF-β-treated AGS cells abolished epithelial-mesenchymal transition with normalization of ZEB1, vimentin and Snail1 expression. Environ Res 2023; 116115. https://doi.org/10.1016/j.envres.2023.116115

Haraguchi M, Sato M, Ozawa M. CRISPR/Cas9n-Mediated Deletion of the Snail 1Gene (SNAI1) Reveals Its Role in Regulating Cell Morphology, Cell-Cell Interactions, and Gene Expression in Ovarian Cancer (RMG-1) Cells. PLoS One 2015; 10(7): e0132260. https://doi.org/10.1371/journal.pone.0132260

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Published

2023-05-26

Issue

Vol. 12 (2023)

Section

Articles

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

How to Cite

Targeting Snail1 by CRISPR/Cas9 System Inhibits the Proliferation and Migration of Human Gastric Cancer Cells. (2023). Journal of Cancer Research Updates, 12, 28-32. https://doi.org/10.30683/1929-2279.2023.12.5

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